2008 – Isopropanol for precipitation of blood proteins prior to small molecule LCMS

T-infusion of deuterium labeled standards shows how ion-suppression occurs during electrospray ionization of metanephrines. Using this type of experiment helps visualize the difference in sensitivity caused by complicated ion-suppression phenomena. Ion suppression is a fantastic and interesting phenomena, read more about it from Tom Annesley, who among other things, is a fantastic science writer: http://www.clinchem.org/content/49/7/1041.long

Isopropanol protein precipitation for the analysis of plasma free metanephrines by liquid chromatography-tandem mass spectrometry.

Marney LC, Laha TJ, Baird GS, Rainey PM, Hoofnagle AN.

Clin Chem. 2008 Oct;54(10):1729-32. doi: 10.1373/clinchem.2008.104083. Epub 2008 Aug 7.

PMID:
18687739

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